Fibroblast Radiosensitivity and Clonogenic Survival
Author Information
Author(s): Eastham A M, Marples B, Kiltie A E, Orton C J, West C M L
Primary Institution: Cancer Research Campaign Section of Genome Damage and Repair, Paterson Institute for Cancer Research
Hypothesis
The study investigates whether fibroblast radiosensitivity measured by the comet assay correlates with clonogenic survival parameters.
Conclusion
The study found that fibroblast radiosensitivity measured using the neutral comet assay correlates with clonogenic radiation survival parameters.
Supporting Evidence
- A significant correlation was found between the extent of residual DNA double strand breaks and clonogenicity for all 11 fibroblast strains.
- The ratio of initial/residual DNA double strand break dose–response slope showed a high correlation with fibroblast cell killing.
Takeaway
The researchers looked at how sensitive certain cells are to radiation and found a way to measure it that might help predict how normal tissues react to radiation treatment.
Methodology
The study used the neutral comet assay to measure DNA double strand breaks in fibroblast strains and correlated these with clonogenic survival parameters.
Participant Demographics
Nine fibroblast strains from cervical cancer patients and two from radiosensitive individuals.
Statistical Information
P-Value
< 0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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