Genotyping of β-Lactoglobulin Gene in Sahiwal and Tharparkar Cattle
Author Information
Author(s): Rachagani Satyanarayana, Gupta Ishwar Dayal, Gupta Neelam, Gupta SC
Primary Institution: National Dairy Research Institute
Hypothesis
The study aims to identify the allelic variants of the β-Lactoglobulin gene in Sahiwal and Tharparkar cattle breeds.
Conclusion
The study found that the allele B of β-Lactoglobulin occurred at a higher frequency than allele A in both Sahiwal and Tharparkar breeds.
Supporting Evidence
- The allele B of β-Lactoglobulin occurred at a higher frequency than allele A in both breeds.
- Genotypic frequencies of AA, AB, and BB were 0.031, 0.276, and 0.693 in Sahiwal, and 0.023, 0.733, and 0.244 in Tharparkar.
- Frequencies of A and B alleles were 0.17 and 0.83 in Sahiwal, and 0.39 and 0.61 in Tharparkar.
Takeaway
This study looked at two types of cows and found that one type of milk protein is more common than another in both breeds.
Methodology
Blood samples were collected, DNA was extracted, and genotyping was performed using PCR-RFLP analysis.
Limitations
The Tharparkar population was not in Hardy-Weinberg equilibrium due to continuous migration of animals.
Participant Demographics
228 Sahiwal and 86 Tharparkar cattle were analyzed.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
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