Effects of High-Fat Diets on Mice with Different Body Types
Author Information
Author(s): Karin Nuernberg, Bernhard H. Breier, Shakeela N. Jayasinghe, Hannes Bergmann, Nichola Thompson, Gerd Nuernberg, Dirk Dannenberger, Falk Schneider, Ulla Renne, Martina Langhammer, Korinna Huber
Primary Institution: Leibniz Institute for Farm Animal Biology
Hypothesis
Mice selected for either high body weight or leanness show different responses in lipid metabolism and alterations in insulin effectiveness.
Conclusion
A diet high in n-3 PUFA may shift fuel storage from the liver to adipose tissue in mice, with varying effects based on genetic predisposition.
Supporting Evidence
- DU6 mice showed higher plasma leptin and insulin levels compared to DUhTP mice.
- High-fat diets increased body fat only in DU6 mice.
- n-3 HFD reduced hepatic insulin receptor β protein concentration in both mouse lines.
- n-3 HFD increased PKC ζ expression in adipose tissue of DUhTP mice, indicating enhanced insulin sensitivity.
Takeaway
This study looked at how different diets affect mice that are either heavy or lean, finding that the type of fat in their food changes how their bodies store energy.
Methodology
The study involved feeding two lines of mice (DU6 and DUhTP) different high-fat diets for 8 weeks and measuring their metabolic responses.
Limitations
Differences in protein content of the control diet and high-fat diets could have influenced metabolic processes.
Participant Demographics
60 male mice from two different selection lines.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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