Five Quantitative Trait Loci Control Radiation-Induced Adenoma Multiplicity in Mom1R ApcMin/+ Mice
Author Information
Author(s): Elahi Eiram, Suraweera Nirosha, Volikos Emmanouil, Haines Jackie, Brown Natalie, Davidson Gerovie, Churchman Mike, Ilyas Mohammed, Tomlinson Ian, Silver Andrew
Primary Institution: Colorectal Cancer Genetics, Institute for Cell and Molecular Sciences, Barts and The London, Queen Mary's School of Medicine and Dentistry, London, United Kingdom
Hypothesis
The study aims to identify quantitative trait loci (QTLs) that influence radiation-induced tumor multiplicity in ApcMin/+ mice.
Conclusion
The study identified five significant QTLs associated with increased radiation-induced intestinal tumorigenesis in ApcMin/+ mice.
Supporting Evidence
- Five significant susceptibility quantitative trait loci (QTLs) were identified for radiation-induced tumor multiplicity.
- Interval and composite interval mapping along with permutation testing were used to identify QTLs.
- Two highly significant QTLs were found on chromosome 5, and one significant QTL on chromosome 2.
Takeaway
Scientists found specific areas in the mouse genome that affect how many tumors develop after radiation exposure.
Methodology
The study involved backcrossing ApcMin/+ mice with BALB/c mice, irradiating them, and then genotyping and phenotyping to identify QTLs.
Limitations
The study may not account for all genetic factors influencing tumor multiplicity due to the specific mouse strains used.
Participant Demographics
The study involved male and female N2 ApcMin/+ mice, specifically 84 males and 58 females irradiated, and 60 males and 51 females sham-irradiated.
Statistical Information
P-Value
p<0.00001
Statistical Significance
p<0.00001
Digital Object Identifier (DOI)
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