GAPDH Regulation in Glioblastoma Under Hypoxia
Author Information
Author(s): Said Harun M, Carsten Hagemann, Jelena Stojic, Beate Schoemig, Giles H Vince, Michael Flentje, Klaus Roosen, Dirk Vordermark
Primary Institution: University of Würzburg, Dept. of Radiation Oncology, Germany
Hypothesis
Is GAPDH expression regulated by hypoxia in human glioblastoma cells?
Conclusion
GAPDH is not regulated under hypoxic conditions in glioblastoma, making it a reliable housekeeping gene but not a suitable therapeutic target.
Supporting Evidence
- GAPDH expression was similar in low-grade astrocytoma and glioblastoma samples.
- No hypoxia-induced regulatory effect on GAPDH expression was observed in glioblastoma cell lines.
- GAPDH is a reliable housekeeping gene for experiments involving hypoxia.
Takeaway
GAPDH, a gene often used to measure other genes, doesn't change when there's low oxygen in brain tumors, so it's a good choice for experiments.
Methodology
GAPDH and β-actin expression were examined in glioblastoma cell lines and tumor samples using Western blot and semiquantitative RT-PCR.
Limitations
The study did not measure direct oxygenation levels in human tumors.
Participant Demographics
11 patients with glioblastoma and 11 patients with low-grade astrocytoma.
Digital Object Identifier (DOI)
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