Distinct roles of Shh and Fgf signaling in regulating cell proliferation during zebrafish pectoral fin development
2008
How Shh and Fgf Signaling Affect Zebrafish Fin Development
publication
Evidence: high
Author Information
Author(s): Sergey V Prykhozhij, Carl J Neumann
Primary Institution: European Molecular Biology Laboratory
Hypothesis
What are the distinct roles of Shh and Fgf signaling in regulating cell proliferation during zebrafish pectoral fin development?
Conclusion
Fgf signaling is crucial for directing proliferation in zebrafish fin buds, while Shh's role is indirect and mediated through Fgf signaling.
Supporting Evidence
- Fgf signaling is sufficient to direct proliferation in zebrafish fin buds in the absence of Shh.
- Inhibition of Shh signaling leads to a loss of cell-cycle gene expression after a delay, correlating with a loss of Fgf signaling.
- Fgf signaling affects cell-cycle progression rapidly, while Shh's effect is indirect.
Takeaway
This study shows that Fgf helps cells grow in zebrafish fins, while Shh helps Fgf do its job but doesn't directly make the cells grow.
Methodology
The study used genetic and pharmacological methods in zebrafish to analyze the effects of Shh and Fgf signaling on cell proliferation.
Digital Object Identifier (DOI)
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