Resveratrol prevents antibody-induced apoptotic death of retinal cells through upregulation of Sirt1 and Ku70
2008
Resveratrol Protects Retinal Cells from Death
publication
Evidence: moderate
Author Information
Author(s): Anekonda Thimmappa S, Adamus Grazyna
Primary Institution: Oregon Health & Science University
Hypothesis
Resveratrol upregulates Sirt1 and Ku70 in retinal cells and suppresses Bax in the cytoplasm, therefore protecting cells from apoptotic death induced by anti-retinal antibody.
Conclusion
Resveratrol protects retinal cells from apoptosis by reducing intracellular calcium levels and regulating pro-apoptotic and anti-apoptotic proteins.
Supporting Evidence
- Resveratrol-treated cells showed 30-55% more survival compared to untreated cells.
- Western blotting indicated reduced Bax levels in resveratrol-treated cells.
- Resveratrol increased Sirt1 expression about five times in treated cells.
Takeaway
Resveratrol is like a superhero for retinal cells, helping them stay alive when bad antibodies try to make them die.
Methodology
E1A.NR3 retinal cells were treated with resveratrol and then exposed to antibodies to measure cell survival and protein expression.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website