Septins and Bacterial Invasion
Author Information
Author(s): Mostowy Serge, Nam Tham To, Danckaert Anne, Guadagnini Stéphanie, Boisson-Dupuis Stéphanie, Pizarro-Cerdá Javier, Cossart Pascale
Primary Institution: Institut Pasteur
Hypothesis
Do septins play a role in the entry of invasive bacteria into non-phagocytic mammalian cells?
Conclusion
SEPT2 is critical for the bacterial entry process and regulates InlB/Met-mediated entry of Listeria into host cells.
Supporting Evidence
- SEPT2-depletion by siRNA decreased bacterial invasion.
- SEPT2 is required for the appropriate signaling cascade in response to Met stimulation by InlB.
- 75% of actin-positive Listeria had collars of SEPT2, SEPT9, or SEPT11 in JEG-3 cells.
Takeaway
Septins are proteins that help bacteria get into cells, and one of them, SEPT2, is really important for this process.
Methodology
The study used siRNA to deplete SEPT2 and assessed its impact on bacterial invasion using gentamicin survival assays.
Limitations
The study primarily focused on two cell lines, which may limit the generalizability of the findings.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
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