How Notch Signaling Affects Inflammation in Sepsis
Author Information
Author(s): Tsao Po-Nien, Wei Shu-Chen, Huang Miao-Tzu, Lee Ming-Cheng, Chou Hung-Chieh, Chen Chien-Yi, Hsieh Wu-Shiun
Primary Institution: National Taiwan University Hospital
Hypothesis
Notch signaling may play a role in the pathogenesis of sepsis.
Conclusion
Notch inhibitors may be useful as adjunctive agents for treating sepsis syndrome.
Supporting Evidence
- Notch signaling was activated after LPS stimulation.
- Disruption of Notch signaling by DAPT reduced inflammatory responses.
- DAPT treatment improved survival in experimental sepsis models.
Takeaway
This study shows that a signaling pathway called Notch helps control inflammation during sepsis, and blocking it can help improve survival in mice.
Methodology
The study used real-time PCR and western blotting to analyze Notch signaling in LPS-stimulated murine macrophages and tested the effects of a Notch inhibitor in vitro and in vivo.
Limitations
The protective effect of the Notch inhibitor DAPT was only partial in vivo.
Participant Demographics
Murine macrophage cell line and C56BL/6 strain mice were used.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website