Effect of a Standardised Dietary Restriction Protocol on Multiple Laboratory Strains of Drosophila melanogaster
2009

Dietary Restriction and Lifespan in Fruit Flies

Sample size: 100 publication 10 minutes Evidence: high

Author Information

Author(s): Richard C. Grandison, Richard Wong, Timothy M. Bass, Linda Partridge, Matthew D. W. Piper

Primary Institution: UCL Institute of Healthy Ageing, University College London

Hypothesis

Does dietary restriction extend lifespan in Drosophila melanogaster despite variations in laboratory conditions?

Conclusion

Dietary restriction genuinely extends lifespan in Drosophila, unaffected by stock maintenance, genotype differences, or microbial infection.

Supporting Evidence

  • Different laboratory strains of Drosophila exhibited varying lifespans under the same dietary conditions.
  • Intermittent feeding did not extend lifespan in Drosophila, suggesting that nutrient restriction is critical.
  • Tetracycline treatment did not eliminate the dietary restriction response in Drosophila.

Takeaway

If fruit flies eat less food, they can live longer, and this is true no matter how they are kept in the lab.

Methodology

The study involved testing different dietary restriction protocols on various Drosophila strains to assess their impact on lifespan and fecundity.

Potential Biases

Potential biases from laboratory conditions affecting lifespan outcomes were acknowledged.

Limitations

The study's findings may not apply to all strains of Drosophila or other organisms due to genetic and environmental differences.

Participant Demographics

The study focused on multiple laboratory strains of Drosophila melanogaster, including Dahomey, OregonR, and CantonS.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.001

Digital Object Identifier (DOI)

10.1371/journal.pone.0004067

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