Overexpression of α(1,3)-fucosyltransferase VII is sufficient for the acquisition of lung colonization phenotype in human lung adenocarcinoma HAL-24Luc cells
1999
Lung Cancer Cells and Fucosyltransferase VII
publication
Evidence: moderate
Author Information
Author(s): Martín-Satué M, Castellarnau C de, Blanco J
Primary Institution: Institut de Recerca Oncològica
Hypothesis
Overexpression of α(1,3)-fucosyltransferase VII is sufficient for the acquisition of lung colonization phenotype in human lung adenocarcinoma HAL-24Luc cells.
Conclusion
Fucosyltransferase VII overexpression in HAL-24Luc cells enables them to adhere to endothelial cells and acquire a lung colonization phenotype.
Supporting Evidence
- Metastatic human lung adenocarcinoma HAL-8Luc cells show enhanced expression of α(1,3)-fucosyltransferases compared to non-metastatic HAL-24Luc cells.
- Fuc-TVII transfectants exclusively overexpress sialyl-Lex, while Fuc-TIV transfected cells only overexpress Lex oligosaccharide.
- Fuc-TVII transfectants can adhere to interleukin-1β-stimulated HUVEC monolayers.
Takeaway
When certain cells in lung cancer have more of a specific protein, they can stick better to blood vessels and spread to the lungs.
Methodology
The study involved stably transfecting HAL-24Luc cells with cDNAs for α(1,3)-Fuc-TIV and VII enzymes and analyzing their expression using flow cytometry.
Digital Object Identifier (DOI)
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