Development of Microsatellite Markers in White Clover
Author Information
Author(s): Zhang Yan, He Ji, Zhao Patrick X, Bouton Joseph H, Monteros Maria J
Primary Institution: The Samuel Roberts Noble Foundation
Hypothesis
The study aims to develop a new approach for microsatellite marker development in white clover.
Conclusion
The combined approach of identifying SSR-enriched fragments by FIASCO and using phpSSRMiner for primer design is an efficient method for developing microsatellite markers in plants.
Supporting Evidence
- 6,816 clones were generated from two SSR-enriched libraries.
- 191 SSR primers were tested for polymorphism, with 92% producing amplicons.
- 66% of the tested SSR primers were polymorphic.
Takeaway
The researchers found a way to create markers that help scientists study white clover better, which can help improve this important plant.
Methodology
The study used FIASCO to create genomic libraries and phpSSRMiner for primer design, followed by testing the polymorphism of 191 SSR markers.
Limitations
The study primarily focuses on white clover and may not be directly applicable to other species without modification.
Digital Object Identifier (DOI)
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