Tools for Analyzing Pyrophosphate Sequencing Data
Author Information
Author(s): Parker Nicolas J, Parker Andrew G
Primary Institution: International Atomic Energy Agency
Hypothesis
Can simple tools be developed to effectively analyze large datasets from pyrophosphate sequencing?
Conclusion
The tools developed allow users to access additional information from pyrophosphate sequencing data that is otherwise difficult to obtain.
Supporting Evidence
- The tools can search a database of over 300,000 fragments for matches.
- They allow for simple assembly and checking of existing sequences.
- The tools are designed to be user-friendly for those with basic computer skills.
Takeaway
This study created easy-to-use tools that help people analyze DNA sequencing data without needing advanced computer skills.
Methodology
The tools were developed using ISO standard C code compiled under GNU Linux and can be run from a live CD without installing Linux.
Limitations
The tools may not resolve all issues related to the assembly of short reads and are limited by the capabilities of the underlying sequencing technology.
Digital Object Identifier (DOI)
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