Comparison of Three Targeted Enrichment Strategies on the SOLiD Sequencing Platform
Author Information
Author(s): Dale J. Hedges, Toumy Guettouche, Shan Yang, Guney Bademci, Ashley Diaz, Ashley Andersen, William F. Hulme, Sara Linker, Arpit Mehta, Yvonne J. K. Edwards, Gary W. Beecham, Eden R. Martin, Margaret A. Pericak-Vance, Stephan Zuchner, Jeffery M. Vance, John R. Gilbert
Primary Institution: University of Miami Miller School of Medicine
Hypothesis
How do different targeted enrichment methods perform when used with the ABI SOLiD sequencing platform?
Conclusion
Agilent SureSelect showed superior on-target efficiency and coverage depth compared to Nimblegen and Raindance methods.
Supporting Evidence
- Agilent SureSelect exhibited superior on-target efficiency.
- Nimblegen performance was similar at read depths of 20× and below.
- Raindance demonstrated the highest versatility in assay design.
Takeaway
The study tested three ways to focus on specific parts of the genome for sequencing, finding that one method worked better than the others.
Methodology
The study compared three enrichment techniques: Agilent SureSelect, Nimblegen SeqCap, and Raindance PCR, using 18 human samples.
Potential Biases
Potential biases in allele representation due to enrichment and sequencing processes were noted.
Limitations
The study was limited to a specific set of genomic regions and may not generalize to other regions or methods.
Participant Demographics
11 females and 7 males were included in the study.
Digital Object Identifier (DOI)
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