Phosphatidylserine Coating Enhances Uptake of Carbon Nanotubes by Immune Cells
Author Information
Author(s): Konduru Nagarjun V., Tyurina Yulia Y., Feng Weihong, Basova Liana V., Belikova Natalia A., Bayir Hülya, Clark Katherine, Rubin Marc, Stolz Donna, Vallhov Helen, Scheynius Annika, Witasp Erika, Fadeel Bengt, Kichambare Padmakar D., Star Alexander, Kisin Elena R., Murray Ashley R., Shvedova Anna A., Kagan Valerian E.
Primary Institution: University of Pittsburgh
Hypothesis
Coating single-walled carbon nanotubes (SWCNT) with phosphatidylserine (PS) will enhance their recognition and uptake by professional phagocytes.
Conclusion
PS-coated SWCNT are effectively recognized and taken up by various phagocytic cells, facilitating targeted delivery of therapeutic agents.
Supporting Evidence
- PS-coated SWCNT were taken up more effectively by RAW264.7 macrophages compared to non-coated or PC-coated SWCNT.
- Uptake of PS-coated SWCNT was significantly inhibited by Annexin V, confirming the role of PS in recognition.
- In vivo studies showed that PS-coated SWCNT were preferentially phagocytized by alveolar macrophages.
- PS-coating altered cytokine production in macrophages, promoting anti-inflammatory responses.
Takeaway
The study shows that adding a special signal to carbon nanotubes helps immune cells recognize and absorb them better, which could be useful for delivering medicine.
Methodology
The study involved in vitro and in vivo experiments assessing the uptake of PS-coated SWCNT by various phagocytic cells and measuring cytokine production.
Potential Biases
Potential bias in the interpretation of results due to the specific focus on PS-coated SWCNT and their interactions with selected cell types.
Limitations
The study primarily focuses on specific cell types and may not generalize to all immune cells or conditions.
Participant Demographics
The study involved murine models and human-derived phagocytes.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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