The testis-specific factor CTCFL cooperates with the protein methyltransferase PRMT7 in H19 imprinting control region methylation
2006
CTCFL and PRMT7 in H19 Methylation
publication
Evidence: moderate
Author Information
Author(s): Jelinic Petar, Stehle Jean-Christophe, Shaw Phillip
Primary Institution: Institute of Pathology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland
Hypothesis
Do CTCFL and PRMT7 cooperate in the methylation of the H19 imprinting control region?
Conclusion
CTCFL and PRMT7 work together to induce DNA methylation at the H19 imprinting control region during male germline development.
Supporting Evidence
- CTCFL is expressed during embryonic male germ cell development, coinciding with ICR methylation.
- CTCFL interacts with PRMT7, which is also expressed during testis development.
- In vitro studies show that CTCFL stimulates the histone-methyltransferase activity of PRMT7.
Takeaway
CTCFL and PRMT7 are like a team that helps turn on a special switch in genes that only comes from dad, making sure they work properly.
Methodology
The study used chromatin immunoprecipitation (ChIP) and in vitro assays in Xenopus oocytes to analyze the interactions and effects of CTCFL and PRMT7 on DNA methylation.
Digital Object Identifier (DOI)
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