Novel markers for differentiation of lobular and ductal invasive breast carcinomas by laser microdissection and microarray analysis

2007

Differentiating Lobular and Ductal Breast Carcinomas Using Gene Expression Analysis

Sample size: 30 publication 10 minutes Evidence: moderate

Author Information

Author(s): Turashvili Gulisa, Bouchal Jan, Baumforth Karl, Wei Wenbin, Dziechciarkova Marta, Ehrmann Jiri, Klein Jiri, Fridman Eduard, Skarda Jozef, Srovnal Josef, Hajduch Marian, Murray Paul, Kolar Zdenek

Primary Institution: Palacky University, Olomouc, Czech Republic

Hypothesis

The study aims to identify gene expression profiles of invasive ductal carcinoma (IDC) and invasive lobular carcinoma (ILC) in relation to normal breast epithelial cells.

Conclusion

The study found that IDC and ILC can be differentiated at both gene and protein levels, with specific candidate genes identified that may play significant roles in breast carcinogenesis.

Supporting Evidence

84 named genes were identified that are common to lobular carcinoma versus normal cell types.
74 named genes were identified that are common to ductal carcinoma versus normal cell types.
28 named genes were found to be differentially expressed between IDC and ILC.
Immunohistochemistry confirmed the differential expression of several novel markers.
ASPN and CTHRC1 were identified as candidate genes significant in breast carcinogenesis.
E-cadherin was found to be downregulated in lobular tumors.
Both tumor types showed upregulated genes involved in tumor-ECM interactions.
Further studies with larger patient sets are needed to verify the findings.

Takeaway

Researchers looked at breast cancer samples to see how two types of cancer, lobular and ductal, are different. They found some special genes that help tell them apart.