Insights into the structure of the CCR4-NOT complex by electron microscopy
2011
Understanding the CCR4-NOT Complex Structure
publication
Evidence: moderate
Author Information
Author(s): Nasertorabi Fariborz, Batisse Claire, Diepholz Meikel, Suck Dietrich, Böttcher Bettina
Primary Institution: European Molecular Biology Laboratory
Hypothesis
What is the three-dimensional structure of the CCR4-NOT complex?
Conclusion
The CCR4-NOT complex is L-shaped and forms an accessible cavity that may facilitate RNA-deadenylation.
Supporting Evidence
- The CCR4-NOT complex was purified from yeast.
- Electron microscopy revealed an L-shaped structure.
- The complex consists of nine proteins.
- Cross-linking improved the stability of the complex.
Takeaway
Scientists studied a protein complex that helps control RNA stability, and they found it has a unique shape that could help it do its job better.
Methodology
The CCR4-NOT complex was purified from yeast and analyzed using electron microscopy and image reconstruction.
Limitations
The study's resolution was limited, and the complex showed inherent instability.
Digital Object Identifier (DOI)
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