Myeloproliferative disorder FOP-FGFR1 fusion kinase recruits phosphoinositide-3 kinase and phospholipase Cγ at the centrosome
2008
FOP-FGFR1 Fusion Kinase and Its Role in Myeloproliferative Disorders
publication
Evidence: moderate
Author Information
Author(s): Lelièvre Hélène, Chevrier Véronique, Tassin Anne-Marie, Birnbaum Daniel
Primary Institution: Centre de Recherche en Cancérologie de Marseille
Hypothesis
The FOP-FGFR1 fusion protein activates the PI3K pathway and recruits substrates at the centrosome, contributing to myeloproliferative disorders.
Conclusion
FOP-FGFR1 recruits and activates PI3K and PLCγ1 at the centrosome, leading to continuous cell growth and myeloproliferative disorders.
Supporting Evidence
- FOP-FGFR1 interacts with CAP350 at the centrosome.
- FOP-FGFR1 activates the PI3K pathway.
- PI3K pathway inhibitors induce death of FOP-FGFR1 expressing cells.
- FOP-FGFR1 recruits PLCγ1 at the centrosome.
Takeaway
A protein called FOP-FGFR1 helps cells grow too much by pulling in helpers at a part of the cell called the centrosome.
Methodology
The study involved experiments with Ba/F3 cells and various molecular biology techniques including immunoprecipitation and western blotting.
Digital Object Identifier (DOI)
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