How Yeast Proteins Mig1, Mig2, and Mig3 Control Gene Expression
Author Information
Author(s): Jakub Westholm, Niklas Nordberg, Eva Murén, Adam Ameur, Jan Komorowski, Hans Ronne
Primary Institution: The Linnaeus Centre for Bioinformatics, Uppsala University
Hypothesis
How do the yeast proteins Mig1, Mig2, and Mig3 contribute to gene regulation during glucose repression?
Conclusion
Mig2 fine-tunes glucose repression by targeting a subset of the Mig1-repressed genes, while Mig3 downregulates the SIR2 gene involved in gene silencing and aging.
Supporting Evidence
- Mig1 and Mig2 repress a largely overlapping set of genes on 2% glucose.
- Most genes upregulated in a mig1 mig2 double mutant show partially redundant repression.
- Mig3 does not overlap in function with Mig1 and Mig2.
Takeaway
This study looks at how three proteins in yeast work together to control which genes are turned on or off when there's sugar around. They found that two of the proteins help each other, while the third one works differently.
Methodology
Microarrays were used to study gene expression in wild type and all combinations of mig1, mig2, and mig3 deletion mutants under glucose conditions.
Limitations
The study primarily focuses on gene expression under specific glucose conditions and may not account for other environmental factors.
Statistical Information
P-Value
1.5e-28
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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