Bile miR-9 as a Biomarker
Author Information
Author(s): Shigehara Kengo, Yokomuro Shigeki, Ishibashi Osamu, Mizuguchi Yoshiaki, Arima Yasuo, Kawahigashi Yutaka, Kanda Tomohiro, Akagi Ichiro, Tajiri Takashi, Yoshida Hiroshi, Takizawa Toshihiro, Uchida Eiji
Primary Institution: Nippon Medical School, Tokyo, Japan
Hypothesis
Can miRNAs in human bile serve as diagnostic biomarkers for biliary tract cancer?
Conclusion
Bile miRNAs, particularly miR-9, may be useful diagnostic indicators for biliary tract cancer.
Supporting Evidence
- 10 of the 667 miRNAs were significantly more highly expressed in the malignant group than in the benign group.
- miR-9 and miR-145* showed high sensitivity levels for diagnosing biliary tract cancer.
- Bile miRNAs were confirmed to be stable and localized to biliary epithelial cells.
Takeaway
Doctors can find tiny pieces of RNA in bile that might help them tell if someone has a type of cancer called biliary tract cancer.
Methodology
Bile samples were collected from patients, and miRNA expression was analyzed using high-throughput real-time PCR-based microarrays.
Limitations
The study is small and requires validation with larger populations.
Participant Demographics
18 patients with biliary disease, including 9 with malignant and 9 with benign conditions.
Statistical Information
P-Value
p<0.0005
Statistical Significance
p<0.0005
Digital Object Identifier (DOI)
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