Herpes Simplex Virus and INSM1 Interaction
Author Information
Author(s): Kamakura Maki, Goshima Fumi, Luo Chenhong, Kimura Hiroshi, Nishiyama Yukihiro
Primary Institution: Nagoya Graduate School of Medicine
Hypothesis
Does the zinc finger transcription factor INSM1 play a role in the expression and localization of the immediate early protein ICP0 during herpes simplex virus infection?
Conclusion
INSM1 up-regulation enhances HSV-1 replication by binding to the ICP0 promoter.
Supporting Evidence
- INSM1 gene expression increased at least 400-fold after HSV-1 infection.
- INSM1 was shown to bind to the ICP0 promoter.
- INSM1-specific siRNA inhibited HSV-1 replication.
Takeaway
When the herpes virus infects cells, it makes a protein called INSM1 work harder, which helps the virus grow and spread.
Methodology
The study used microarray analysis, RT-PCR, luciferase reporter assays, and ChIP assays to investigate the role of INSM1 in HSV infection.
Limitations
The study did not determine whether the up-regulation of INSM1 mRNA corresponds to the production of functional INSM1 protein.
Participant Demographics
Normal human epidermal keratinocytes and HaCaT cells were used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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