Cdx1 and c-Myc Foster the Initiation of Transdifferentiation of the Normal Esophageal Squamous Epithelium toward Barrett's Esophagus
2008

Cdx1 and c-Myc in Barrett's Esophagus

Sample size: 7 publication Evidence: moderate

Author Information

Author(s): Stairs Douglas B., Nakagawa Hiroshi, Klein-Szanto Andres, Mitchell Shukriyyah D., Silberg Debra G., Tobias John W., Lynch John P., Rustgi Anil K.

Primary Institution: University of Pennsylvania

Hypothesis

Do Cdx1 and c-Myc contribute to the transdifferentiation of normal esophageal cells toward Barrett's esophagus?

Conclusion

Cdx1 and c-Myc can initiate the early stages of transdifferentiation of esophageal keratinocytes toward Barrett's esophagus.

Supporting Evidence

  • Cdx1 and c-Myc were identified as key factors in the transdifferentiation process.
  • Microarray analysis showed significant gene expression changes in Barrett's esophagus.
  • Cdx1 and c-Myc cooperatively induced mucin production in esophageal keratinocytes.

Takeaway

This study found that two proteins, Cdx1 and c-Myc, help normal esophageal cells change into a type that can lead to Barrett's esophagus, which is a condition that can cause cancer.

Methodology

Microarray analysis of biopsy specimens from normal esophagus, Barrett's esophagus, and small intestine was performed to identify genetic changes.

Potential Biases

Potential bias due to the limited number of biopsy samples and the specific patient population.

Limitations

The study may not detect all gene profile changes with a single biopsy sample per patient.

Participant Demographics

Patients undergoing upper endoscopy at the Hospital of the University of Pennsylvania.

Statistical Information

P-Value

5×10−5

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0003534

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