Detecting a Rare AKT1 Mutation in Lung Cancer
Author Information
Author(s): Do Hongdo, Solomon Benjamin, Mitchell Paul L, Fox Stephen B, Dobrovic Alexander
Primary Institution: Peter MacCallum Cancer Centre
Hypothesis
Is the AKT1 E17K mutation present in non-small cell lung cancer (NSCLC)?
Conclusion
The AKT1 E17K mutation is very rare in lung cancer and might be associated with tumorigenesis in squamous cell carcinoma.
Supporting Evidence
- The E17K mutation was found in one of the 14 squamous cell carcinomas.
- No mutations were found in 141 adenocarcinomas and 39 large cell carcinomas.
- HRM is a rapid and cost-effective method for screening low frequency mutations.
Takeaway
Scientists looked for a rare mutation in lung cancer samples and found it in only one case, suggesting it's not common in this type of cancer.
Methodology
High resolution melting (HRM) analysis was used to screen 219 NSCLC samples for the AKT1 E17K mutation.
Limitations
The study only identified the mutation in one sample, indicating a need for further research to confirm its frequency.
Participant Demographics
The tumor sample with the mutation was from a female diagnosed with NSCLC at the age of seventy.
Digital Object Identifier (DOI)
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