Changes in Na+/K+-ATPase in Rabbit Lacrimal Glands During Autoimmune Dacryoadenitis
Author Information
Author(s): Ding Chuanqing, Lu Michael, Huang Jianyan
Primary Institution: Keck School of Medicine, University of Southern California
Hypothesis
The expression of Na+/K+-ATPase subunits in the lacrimal glands of rabbits with induced autoimmune dacryoadenitis changes.
Conclusion
Significant changes in mRNA and protein expressions of Na+/K+-ATPase subunits in rabbits with autoimmune dacryoadenitis suggest a role in the pathogenesis of Sjögren’s syndrome and altered lacrimal gland secretion.
Supporting Evidence
- mRNA levels of α1, β1, and β3 were significantly lower in rabbits with autoimmune dacryoadenitis compared to controls.
- Western blots showed that all five Na+/K+-ATPase subunits were significantly higher in rabbits with autoimmune dacryoadenitis.
- Changes in Na+/K+-ATPase expression may contribute to reduced tear secretion in rabbits with autoimmune dacryoadenitis.
Takeaway
The study found that the levels of certain proteins in the tear glands of rabbits with a specific eye condition were different from those in healthy rabbits, which might help explain why they have dry eyes.
Methodology
Lacrimal glands were obtained from rabbits with induced autoimmune dacryoadenitis and control rabbits, processed for various analyses including real-time RT-PCR and western blot.
Limitations
The study primarily focused on mRNA and protein levels without direct functional studies to confirm the physiological implications.
Participant Demographics
Adult female New Zealand White rabbits.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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