DNA Methylation Changes in Mice Due to Diet
Author Information
Author(s): Boon Kathy, Tomfohr John K, Bailey Nathaniel W, Garantziotis Stavros, Li Zhuowei, Brass David M, Maruoka Shuichiro, Hollingsworth John W, Schwartz David A
Primary Institution: National Heart Lung and Blood Institute/National Institute of Environmental Health Sciences
Hypothesis
Can Methylation Specific Digital Karyotyping (MSDK) effectively analyze epigenetic changes in mice after perinatal nutritional modifications?
Conclusion
The study found that perinatal dietary changes in methyl-donor content can lead to subtle changes in DNA methylation in mice.
Supporting Evidence
- The MSDK method identified 158 loci that were significantly differentially methylated after dietary changes.
- Quantitative PCR confirmed changes in methylation at specific loci.
- The study demonstrated a correlation between methylation changes and transcription levels.
Takeaway
Mice that ate different diets during pregnancy showed changes in their DNA that could affect how their genes work.
Methodology
The study used Methylation Specific Digital Karyotyping (MSDK) to analyze DNA from lung tissue of mice exposed to different diets.
Limitations
The study's findings may be influenced by the mixed cell population in the samples.
Participant Demographics
C57BL/6J adult male mice were used in the study.
Statistical Information
P-Value
0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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