Arf6 recruits the Rac GEF Kalirin to the plasma membrane facilitating Rac activation
2007
Arf6 recruits Kalirin to activate Rac
publication
Evidence: moderate
Author Information
Author(s): Koo Tae Hyeon, Eipper Betty A, Donaldson Julie G
Primary Institution: NHLBI, NIH
Hypothesis
How does Arf6 influence Rac function?
Conclusion
Arf6 can regulate Rac activation by recruiting Kalirin to the plasma membrane.
Supporting Evidence
- Arf6-GDP binds to Kalirin through spectrin repeats.
- Kalirin5 enhances Rac1 activation when co-expressed with wild type Arf6.
- Expression of Arf6T27N inhibits Kalirin5-induced ruffling.
Takeaway
Arf6 helps another protein, Kalirin, to do its job better, which is to activate Rac, a protein that helps cells move.
Methodology
Binding assays and co-immunoprecipitation were used to study the interactions between Arf6 and Kalirin.
Limitations
The interaction was not detectable with endogenous proteins, possibly due to low expression levels.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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