Molecular epidemiology of pneumococci obtained from Gambian children aged 2–29 months with invasive pneumococcal disease during a trial of a 9-valent pneumococcal conjugate vaccine
2008

Molecular Epidemiology of Pneumococci in Gambian Children

Sample size: 132 publication Evidence: moderate

Author Information

Author(s): Antonio Martin, Dada-Adegbola Hannah, Biney Ekow, Awine Tim, O'Callaghan John, Pfluger Valentin, Enwere Godwin, Okoko Brown, Oluwalana Claire, Vaughan Adeola, Zaman Syed MA, Pluschke Gerd, Greenwood Brian M, Cutts Felicity, Adegbola Richard A

Primary Institution: Medical Research Council Laboratories, Banjul, The Gambia

Hypothesis

What is the genetic structure of Streptococcus pneumoniae causing invasive disease in Gambian children?

Conclusion

The study provides important background data on the genetic structure of S. pneumoniae prior to the use of the 9-valent pneumococcal conjugate vaccine in The Gambia.

Supporting Evidence

  • Twenty-nine different serotypes of pneumococci were identified among the isolates.
  • Serotypes 14, 19A, 12F, 5, 23F, and 1 accounted for 58.3% of all serotypes.
  • MLST analysis revealed 72 sequence types, with 46 being novel.

Takeaway

The study looked at germs that cause serious illness in young children in The Gambia and found many different types of these germs.

Methodology

The study analyzed 132 S. pneumoniae isolates from children aged 2–29 months using serotyping, antibiotic susceptibility testing, BOX-PCR, and MLST.

Limitations

The study only included isolates from a specific time period and location, which may not represent the broader population.

Participant Demographics

Children aged 2–29 months from The Gambia, both vaccinated and unvaccinated.

Statistical Information

Confidence Interval

95% CI 157, 715 for children aged 6 weeks to 5 months; 95% CI 369, 857 for those aged 6–11 months.

Digital Object Identifier (DOI)

10.1186/1471-2334-8-81

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