High Light Induced Disassembly of Photosystem II Supercomplexes in Arabidopsis Requires STN7-Dependent Phosphorylation of CP29
2011

How High Light Affects Photosystem II in Plants

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Author Information

Author(s): Rikard Fristedt, Alexander V. Vener

Primary Institution: Linköping University, Linköping, Sweden

Hypothesis

Disassembly of PSII supercomplexes in plants exposed to high light involves STN7-kinase-dependent phosphorylation of the linker protein CP29.

Conclusion

High light treatment causes the relocation of CP29 from PSII supercomplexes to PSII dimers and monomers, which is dependent on STN7 kinase activity.

Supporting Evidence

  • High light treatment increased phosphorylation of PSII core proteins.
  • CP29 relocation from PSII supercomplexes was reversible when plants were returned to normal light.
  • Phosphorylation of CP29 was significantly reduced in stn7 and stn7stn8 mutants.

Takeaway

When plants get too much sunlight, a special protein helps them move parts around to stay healthy. If this protein doesn't work, the plants can get sick.

Methodology

The study analyzed the phosphoproteome of thylakoid membranes in Arabidopsis plants exposed to high light using mass spectrometry and blue native gel electrophoresis.

Limitations

The study primarily focuses on Arabidopsis and may not be generalizable to other plant species.

Participant Demographics

Arabidopsis thaliana wild type and various mutant strains.

Digital Object Identifier (DOI)

10.1371/journal.pone.0024565

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