Evaluation of the toxicity of perfluorooctanoic acid toward human colorectal cancer cells using multi-dimensional approaches
2025
Toxicity of Perfluorooctanoic Acid on Human Colorectal Cancer Cells
Sample size: 4
publication
Evidence: moderate
Author Information
Author(s): ZHANG Ruijia, LIN Yingshi, TU Lanyin, CHEN Zitong, ZHANG Weiwei, LUAN Tiangang, CHEN Baowei
Primary Institution: Hainan University
Hypothesis
What are the toxic effects of perfluorooctanoic acid (PFOA) on human colorectal cancer cells (HCT116)?
Conclusion
High concentrations of PFOA significantly inhibit the activity of HCT116 cells and alter the expression of metabolism-related genes.
Supporting Evidence
- High concentrations of PFOA (300 μmol/L) significantly inhibited HCT116 cell activity.
- Low concentrations of PFOA (50 μmol/L) improved mitochondrial respiration in HCT116 cells.
- DPEP1 and SPHK1 gene expressions were significantly upregulated in response to high PFOA exposure.
Takeaway
This study found that too much PFOA can hurt cancer cells, but a little bit can actually help them breathe better.
Methodology
The study assessed the toxicity of PFOA on HCT116 cells through cytotoxicity assays, mitochondrial respiration analysis, and qPCR for gene expression.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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