MT1-MMP regulates urothelial cell invasion via transcriptional regulation of Dickkopf-3
2008
MT1-MMP and its Role in Urothelial Cell Invasion
publication
Evidence: high
Author Information
Author(s): Saeb-Parsy K, Veerakumarasivam A, Wallard M J, Thorne N, Kawano Y, Murphy G, Neal D E, Mills I G, Kelly J D
Primary Institution: Cancer Research UK Cambridge Research Institute
Hypothesis
MT1-MMP regulates urothelial cell invasion via transcriptional regulation of Dickkopf-3.
Conclusion
MT1-MMP is essential for the invasion of urothelial cell carcinoma cell lines and modulates DKK3 levels, which inhibit invasion.
Supporting Evidence
- MT1-MMP expression is highest in tumours invading the lamina propria and detrusor muscle.
- Knockdown of MT1-MMP led to a 70% reduction in cell invasion.
- DKK3 expression was upregulated following MT1-MMP knockdown, indicating transcriptional regulation.
Takeaway
MT1-MMP helps cancer cells invade, and when we block it, a protein called DKK3 increases, which stops the cells from invading.
Methodology
The study used siRNA to suppress MT1-MMP in urothelial cell carcinoma cell lines and assessed changes in gene expression and invasion potential.
Statistical Information
P-Value
p<0.0005
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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