Drug Resistance in CHO Cells from X-rays and Vincristine
Author Information
Author(s): S. McClean, B.T. Hill
Primary Institution: Cellular Chemotherapy Laboratory, Imperial Cancer Research Fund
Hypothesis
How does exposure to X-rays and vincristine affect drug resistance in Chinese hamster ovary cells?
Conclusion
The study found that further drug selection with vincristine increased drug resistance in CHO cells previously exposed to X-rays.
Supporting Evidence
- DXR-10/VCR-10 cells showed increased resistance to vincristine compared to DXR-10II cells.
- P-glycoprotein levels were significantly higher in DXR-10/VCR-10 cells.
- The study confirmed that drug selection can enhance drug resistance beyond what is achieved with X-ray exposure alone.
Takeaway
When certain cells are treated with X-rays and then with a drug called vincristine, they become better at resisting that drug. This shows that the way cells resist drugs can change based on how they are treated.
Methodology
The study involved treating CHO cells with X-rays and vincristine, followed by assessing their drug resistance and P-glycoprotein expression.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Want to read the original?
Access the complete publication on the publisher's website