WSSV ie1 Promoter is Better for Chicken Vaccine Production
Author Information
Author(s): He Fang, Ho YuenFern, Yu Li, Kwang Jimmy
Primary Institution: Temasek Life Sciences Laboratory, National University of Singapore
Hypothesis
Can the WSSV ie1 promoter enhance the expression of H5 hemagglutinin in baculovirus for use as a chicken vaccine compared to the CMV promoter?
Conclusion
The WSSV ie1 promoter significantly improves the production of H5 hemagglutinin in baculovirus, leading to a stronger immune response in vaccinated chickens.
Supporting Evidence
- WSSV ie1 promoter showed significantly higher luciferase activity in SF9 cells compared to CMV promoter.
- Immunization with baculovirus under WSSV ie1 promoter elicited higher levels of anti-HA antibodies in chickens.
- The HA content produced by WSSV ie1 promoter was significantly higher than that produced by CMV promoter.
Takeaway
This study shows that using a specific virus promoter can help make better vaccines for chickens, which can help protect them from bird flu.
Methodology
Recombinant baculoviruses were constructed to express H5 hemagglutinin under WSSV ie1 and CMV promoters, and their expression efficiency was evaluated in SF9 cells and in immunized chickens.
Limitations
The study primarily focused on chickens, and further research is needed to evaluate the vaccine's effectiveness in other species.
Participant Demographics
Chickens, specifically 2-week-old chicks were used for immunization.
Statistical Information
P-Value
p<0.005
Confidence Interval
95% confidence interval is between 8.72 and 35.02
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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