The Role of Transglutaminase 2 in Arterial Remodeling
Author Information
Author(s): Jeroen van den Akker, Ed VanBavel, Remon van Geel, Hanke L. Matlung, Tuna Bilge Guvenc, George M. C. Janssen, Peter A. van Veelen, Wilbert C. Boelens, Jo G. R. De Mey, Erik N. T. P. Bakker
Primary Institution: Academic Medical Center, University of Amsterdam
Hypothesis
Does the redox state of transglutaminase 2 (TG2) influence arterial remodeling?
Conclusion
A reduced state activates TG2, leading to inward remodeling of small arteries.
Supporting Evidence
- TG2 activity was significantly increased in the presence of a reducing agent.
- Inward remodeling was inhibited by a TG2 inhibitor and nitric oxide donor.
- Mass spectrometry identified 21 proteins as TG2 cross-linking substrates.
- Calcium ionophore increased TG2 activity but did not induce remodeling.
- Endogenous TG2 activation by DTT led to significant inward remodeling.
Takeaway
When blood flow is low, a special protein called TG2 helps arteries change shape, but it needs a certain environment to work properly.
Methodology
Mice were used to isolate small arteries, which were then treated with various agents to study TG2 activity and arterial remodeling.
Potential Biases
Potential bias in the interpretation of results due to the use of specific inhibitors and agents.
Limitations
The study primarily used mouse models, which may not fully represent human physiology.
Participant Demographics
Four months old male C57Bl/6 mice were used in the experiments.
Statistical Information
P-Value
0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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