Panton-Valentine Leukocidin Does Play a Role in the Early Stage of Staphylococcus aureus Skin Infections: A Rabbit Model
2011

The Role of Panton-Valentine Leukocidin in Staphylococcus aureus Skin Infections in Rabbits

Sample size: 33 publication Evidence: moderate

Author Information

Author(s): Lipinska Urszula, Hermans Katleen, Meulemans Lieve, Dumitrescu Oana, Badiou Cedric, Duchateau Luc, Haesebrouck Freddy, Etienne Jerome, Lina Gerard

Primary Institution: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium

Hypothesis

Does Panton-Valentine leukocidin (PVL) contribute to the pathogenicity of Staphylococcus aureus in skin infections?

Conclusion

The study found that PVL contributes to the severity of skin infections caused by Staphylococcus aureus in rabbits.

Supporting Evidence

  • Rabbits infected with PVL positive strains developed larger lesions than those infected with PVL knockout strains.
  • The study supports the epidemiological link between PVL-producing strains and necrotizing skin infections.
  • Histological analysis showed more pronounced damage in rabbits infected with PVL positive strains.

Takeaway

This study shows that a toxin called PVL makes skin infections from a type of bacteria worse in rabbits.

Methodology

Rabbits were injected with different strains of Staphylococcus aureus, and the development of skin lesions was observed over 14 days.

Potential Biases

Potential bias due to the lack of blinding in lesion evaluation.

Limitations

The evaluation of lesions was not performed blinded.

Participant Demographics

Thirty-three twelve-week-old albino hybrid rabbits of either sex, weighing 2.5–3 kg.

Statistical Information

P-Value

p<0.00096

Statistical Significance

p<0.00096

Digital Object Identifier (DOI)

10.1371/journal.pone.0022864

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