An internal ribosome entry site element directs the synthesis of the 80 kDa isoforms of protein 4.1R
2008
How an Internal Ribosome Entry Site Produces Different Isoforms of Protein 4.1R
publication
Evidence: high
Author Information
Author(s): Eva Lospitao, Carmen M. Pérez-Ferreiro, Altea Gosálbez, Miguel A. Alonso, Isabel Correas
Primary Institution: Departamento de Biología Molecular, Universidad Autónoma de Madrid y Centro de Biología Molecular Severo Ochoa
Hypothesis
Does the 699 nt region between AUG1 and AUG2 play a role in 4.1R mRNA translation?
Conclusion
The study demonstrates that an internal ribosome entry site is responsible for the production of 80 kDa isoforms of protein 4.1R.
Supporting Evidence
- The study shows that both long and short isoforms of protein 4.1R can be produced from the same mRNA.
- Mutational studies confirmed that the short isoforms are synthesized from AUG2, not from proteolytic cleavage.
- An internal ribosome entry site was identified in the 4.1R mRNA that directs the synthesis of the short isoforms.
Takeaway
This study found a special part of the protein's instructions that helps make a smaller version of the protein, which is important for the cell.
Methodology
The study involved in vitro expression analysis of 4.1R cDNAs and transfection into COS-7 cells.
Digital Object Identifier (DOI)
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