An emerging consensus for the structure of EmrE EmrE
2009
Understanding the Structure of EmrE, a Multidrug Transporter
publication
Evidence: high
Author Information
Author(s): Korkhov Vladimir M., Tate Christopher G.
Primary Institution: MRC Laboratory of Molecular Biology
Hypothesis
What is the correct structural arrangement of the EmrE dimer in the membrane?
Conclusion
The study confirms that EmrE functions as an antiparallel dimer in the membrane.
Supporting Evidence
- The cryo-EM structure of EmrE has been called the 'gold standard' for comparison.
- Revised X-ray structures show excellent agreement with the cryo-EM model.
- Biochemical data supports the antiparallel orientation of EmrE monomers.
Takeaway
EmrE is like a special door that helps bacteria get rid of harmful substances, and it works best when two of these doors are facing opposite directions.
Methodology
The study utilized cryoelectron microscopy and X-ray crystallography to analyze the structure of EmrE.
Limitations
The study does not definitively confirm the in vivo oligomeric state of EmrE.
Digital Object Identifier (DOI)
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