Effects of UVB on Protein Changes in Skin Cells
Author Information
Author(s): Perluigi Marzia, Di Domenico Fabio, Blarzino Carla, Foppoli Cesira, Cini Chiara, Giorgi Alessandra, Grillo Caterina, De Marco Federico, Butterfield David A, Schininà Maria E, Coccia Raffaella
Primary Institution: Department of Biochemical Sciences, 'Sapienza' University of Rome
Hypothesis
How does UVB-induced oxidative stress affect protein expression and oxidation in human skin cells?
Conclusion
UVB exposure leads to significant protein oxidation and changes in protein expression in human epithelial keratinocytes.
Supporting Evidence
- Fifteen proteins involved in cytoskeleton integrity and stress response were differentially regulated in UVB-exposed cells.
- Seven proteins related to adhesion and folding were selectively oxidized in UVB-treated cells.
- Proteins like HSP60 and HSP70 were up-regulated in response to UVB exposure.
Takeaway
When skin cells are exposed to UVB light, they change some of their proteins to try to protect themselves, but some proteins also get damaged.
Methodology
A proteomics approach was used to analyze protein expression and identify oxidatively modified proteins in UVB-exposed human epithelial keratinocytes.
Participant Demographics
Normal human epithelial keratinocytes obtained from children's foreskins.
Statistical Information
P-Value
<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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