Fast and Easy Method to Identify Authentic Ophiocordyceps sinensis
Author Information
Author(s): Liu Haibin, Wang Xinyue, Tian Hang, Yuan Yi, Wang Jing, Cheng Yani, Sun Linyao, Chen Hongshuo, Song Xiaoming
Primary Institution: North China University of Science and Technology
Hypothesis
Can a rapid DNA extraction and detection method effectively distinguish authentic Ophiocordyceps sinensis from its adulterants?
Conclusion
The RPA-NAH-LFS method can accurately identify Ophiocordyceps sinensis from counterfeit products within 16 minutes.
Supporting Evidence
- The RPA-NAH-LFS method can detect O. sinensis DNA down to 1.4 ng/μL.
- The method showed no cross-reactivity with common adulterants.
- Results from RPA-NAH-LFS were consistent with PCR-AGE and RT-PCR.
- The entire process can be completed in just 16 minutes.
- The method is user-friendly and does not require complex equipment.
Takeaway
This study created a quick test to check if a mushroom called Ophiocordyceps sinensis is real or fake, which can be done in just 16 minutes.
Methodology
The study used rapid DNA extraction followed by recombinase polymerase amplification (RPA) and nucleic acid hybridization lateral flow strip (NAH-LFS) for detection.
Limitations
The method's effectiveness may vary with different sample types and conditions.
Digital Object Identifier (DOI)
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