High Incidence of Non-Random Template Strand Segregation and Asymmetric Fate Determination In Dividing Stem Cells and their Progeny
2007

Non-Random Chromosome Segregation in Stem Cells

publication Evidence: moderate

Author Information

Author(s): Thomas Rando, Richard Robinson

Hypothesis

Stem cells might be more likely than other cells to direct the older, more pristine, DNA strands into the daughter cell that remained a stem cell.

Conclusion

The study found that muscle stem cells do not randomly segregate their chromosomes during division, but instead preferentially allocate older DNA strands to the stem cell.

Supporting Evidence

  • Muscle stem cells preferentially allocate older DNA strands to the stem cell during division.
  • Cells with newer template strands showed higher expression of the differentiation marker Desmin.
  • The study challenges the conventional wisdom that chromosome segregation is random.

Takeaway

When muscle stem cells divide, they try to keep the older, better DNA for themselves and give the newer, more error-prone DNA to their sibling cell.

Methodology

The authors exposed muscle stem cells to modified nucleotides to visualize DNA strands during replication and mitosis.

Limitations

The study does not clarify whether all older chromosomes or just a large fraction end up together in the same daughter cell.

Digital Object Identifier (DOI)

10.1371/journal.pbio.0050125

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