New human hepatocellular carcinoma (HCC) cell line with highly metastatic potential (MHCC97) and its expressions of the factors associated with metastasis
1999
New Human Liver Cancer Cell Line with High Metastatic Potential
publication
Evidence: moderate
Author Information
Author(s): Tian J, Tang Z Y, Ye S L, Liu Y K, Lin Z Y, Chen J, Xue Q
Primary Institution: Liver Cancer Institute and Zhong-Shan Hospital, Shanghai Medical University
Hypothesis
The study aims to establish a new human hepatocellular carcinoma (HCC) cell line with high metastatic potential.
Conclusion
The newly established MHCC97 cell line exhibits high metastatic potential and retains characteristics of the original tumor.
Supporting Evidence
- The MHCC97 cell line was cultivated for 18 months and subcultured for more than 90 passages.
- The cell line showed an aneuploid pattern with chromosome abnormalities.
- Metastatic lesions in lungs were found after inoculating the cells into nude mice.
Takeaway
Researchers created a new type of liver cancer cell that spreads easily, which can help us understand how liver cancer spreads.
Methodology
The cell line was established from a metastatic model of human HCC in nude mice through alternating cell culture and growth in mice.
Digital Object Identifier (DOI)
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