Down-regulation of GRP78 is associated with the sensitivity of chemotherapy to VP-16 in small cell lung cancer NCI-H446 cells
2008
How Lowering GRP78 Helps Lung Cancer Cells Respond to Chemotherapy
publication
Evidence: moderate
Author Information
Author(s): Wang Yingyan, Wang Wei, Wang Siyan, Wang Jiarui, Shao Shujuan, Wang Qi
Primary Institution: Dalian Medical University
Hypothesis
Can down-regulation of GRP78 enhance the sensitivity of small cell lung cancer cells to chemotherapy?
Conclusion
Lowering GRP78 significantly increases the sensitivity of small cell lung cancer cells to the chemotherapy drug VP-16.
Supporting Evidence
- The expression of GRP78 decreased significantly in cells treated with BAPTA-AM.
- Apoptotic rates in BAPTA-AM treated cells were significantly higher compared to control groups.
- Cell cycle analysis showed a decrease in G1 phase and an increase in S phase in treated cells.
Takeaway
Researchers found that reducing a protein called GRP78 makes lung cancer cells more likely to die when treated with a specific chemotherapy drug.
Methodology
NCI-H446 cells were treated with different concentrations of BAPTA-AM and A23187, followed by VP-16 treatment, and analyzed for GRP78 expression and apoptosis.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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