Differences in Inhibition of Glucocorticoid Receptor by p53 and p73
Author Information
Author(s): Zhang Lili, Nie Linghu, Maki Carl G
Primary Institution: Institute of Medicinal Biotechnology, Peking Union Medical College, Beijing, China; Department of Radiation Oncology, University of Chicago, Chicago, IL 60637, USA
Hypothesis
Do p53 and p73 function similarly or differently in inhibiting glucocorticoid receptor (GR) transcriptional activity?
Conclusion
p53 efficiently inhibits GR transcriptional activity, while p73 is either unable to inhibit GR or slightly increases its activity.
Supporting Evidence
- Both p53 and p73 can bind GR.
- Wild-type p53 efficiently inhibited GR transcriptional activity.
- p73 was either unable to efficiently inhibit GR or increased GR activity slightly.
- Chimeric proteins showed that N- and C-terminal regions of p53 and p73 contribute to their regulation of GR.
Takeaway
This study found that p53 can stop a certain protein called GR from working, but p73 can't do that as well, and sometimes it even helps GR work more.
Methodology
The study involved transfecting Saos-2 cells with various combinations of p53, p73, and GR, and measuring their transcriptional activity using luciferase reporter assays.
Digital Object Identifier (DOI)
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