How Alleles Control IGF2BP1 Gene Expression
Author Information
Author(s): Thomas Brandon J, Rubio Eric D, Krumm Niklas, Broin Pilib Ó, Bomsztyk Karol, Welcsh Piri, Greally John M, Golden Aaron A, Krumm Anton
Primary Institution: University of Washington School of Medicine
Hypothesis
Does CTCF binding and histone modification influence monoallelic expression of the IGF2BP1 gene?
Conclusion
Monoallelic expression of the IGF2BP1 gene is regulated through allele-specific transcriptional elongation rather than CTCF binding.
Supporting Evidence
- 293 genomic loci were identified that are associated with CTCF and histone H3 trimethylated at lysine 9.
- CTCF does not regulate mono- or biallelic IGF2BP1 expression.
- RNA polymerase II is detected on both alleles in B lymphoblasts expressing IGF2BP1 primarily from one allele.
Takeaway
This study shows that sometimes genes only use one of their two copies, and this happens during the process of making RNA, not because of special markers on the DNA.
Methodology
The study used chromatin immunoprecipitation (ChIP-chip) to analyze CTCF binding and histone modifications at the IGF2BP1 gene.
Potential Biases
Potential bias in the selection of cell lines and SNPs analyzed.
Limitations
The study was limited to a specific set of cell lines and may not generalize to all cell types.
Participant Demographics
The study involved individuals from a specific CEPH pedigree.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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