Characterization of the meningococcal DNA glycosylase Fpg involved in base excision repair
2009
Study of Meningococcal DNA Repair Enzyme Fpg
publication
Evidence: moderate
Author Information
Author(s): Tibballs Katrina L, Ambur Ole Herman, Alfsnes Kristian, Homberset Håvard, Frye Stephan A, Davidsen Tonje, Tønjum Tone
Primary Institution: Centre for Molecular Biology and Neuroscience and Institute of Microbiology, University of Oslo
Hypothesis
This study aimed at characterizing the meningococcal Fpg and its role in DNA repair.
Conclusion
Meningococcal Fpg functions are similar to those of prototype Fpg orthologues in other bacterial species.
Supporting Evidence
- The fpg gene contained a DNA uptake sequence mediating efficient transformation of DNA.
- The recombinant N. meningitidis Fpg protein was over-expressed and purified.
- N. meningitidis Fpg was found to remove specific DNA lesions.
- The fpg single mutant showed only a slight increase in phase variation frequency compared to a mismatch repair mutant.
Takeaway
The study looked at a protein in bacteria that helps fix damaged DNA, showing it works like similar proteins in other bacteria.
Methodology
The fpg gene was cloned, and its product was over-expressed and purified for enzymatic activity assessment.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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