Met/HGF receptor and its role in colorectal cancer
Author Information
Author(s): Kitamura S, Kondo S, Shinomura Y, Kanayama S, Miyazaki Y, Kiyohara T, Hiraoka S, Matsuzawa Y
Primary Institution: Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University
Hypothesis
The study investigates the role of met expression on the modulation of apoptosis in colorectal tumors.
Conclusion
Met expression modulates apoptosis through bcl-w expression in colorectal tumors.
Supporting Evidence
- The c-met gene was overexpressed in seven of 12 adenomas and seven of 11 carcinomas.
- The bcl-w, bcl-2, and bcl-xL genes were over-expressed in various adenomas and carcinomas.
- The c-met mRNA level correlated with bcl-w but not with bcl-2 or bcl-xL mRNA levels.
- c-met-antisense oligonucleotides decreased Met protein levels in the LoVo human colon cancer cell line.
- Apoptotic cell death was more prominent in c-met-antisense-treated cells compared to controls.
Takeaway
This study found that a specific protein called Met helps control cell death in colorectal cancer, which could be important for understanding how to treat this disease.
Methodology
Gene expressions of c-met and bcl family members were analyzed using quantitative polymerase chain-reaction combined with reverse transcription.
Participant Demographics
The study involved human colorectal adenomas and adenocarcinomas.
Digital Object Identifier (DOI)
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