Modeling Cell Cultivation for Monoclonal Antibody Production
Author Information
Author(s): Okamura Kozue, Badr Sara, Ichida Yusuke, Yamada Akira, Sugiyama Hirokazu
Primary Institution: The University of Tokyo
Hypothesis
How do variations in dissolved oxygen and glutamine depletion affect cell metabolism in monoclonal antibody production?
Conclusion
The developed mechanistic model provides insights into lactate metabolic shifts and can optimize monoclonal antibody production processes.
Supporting Evidence
- The model incorporates variations in dissolved oxygen and glutamine depletion.
- High productivity was achieved with the novel CHO-MK cell line.
- The model can be used for process performance evaluation in terms of productivity and quality.
Takeaway
This study created a model to help understand how cells behave when making antibodies, especially when nutrients change.
Methodology
The study used a mechanistic model to analyze cell metabolism under varying conditions of dissolved oxygen and glutamine.
Limitations
More experiments are needed to validate the model, especially for stirred-tank reactors.
Digital Object Identifier (DOI)
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