Effects of Mdivi-1 on Inflammation and Foam Cell Formation in Monocytes
Author Information
Author(s): Mosalmanzadeh Negin, Maurmann Rafael Moura, Davis Kierstin, Schmitt Brenda Landvoigt, Makowski Liza, Pence Brandt D.
Primary Institution: College of Health Sciences, University of Memphis
Hypothesis
OxLDL would lead to glycolytic reprogramming and proinflammatory responses in monocytes, and that Mdivi-1 would reduce or prevent these responses.
Conclusion
Mdivi-1 effectively reduces the metabolic shift towards glycolysis induced by OxLDL, decreases foam cell formation, and lowers gene expression of inflammatory cytokines.
Supporting Evidence
- Mdivi-1 reduced OxLDL-induced glycolytic reprogramming.
- Mdivi-1 decreased expression of inflammatory cytokines IL1B and CXCL8.
- Mdivi-1 limited foam cell formation in monocytes.
- OxLDL treatment increased glycolytic activity in monocytes.
- Both Mdivi-1 and S1QEL modulated key monocyte responses to OxLDL.
Takeaway
This study shows that a compound called Mdivi-1 can help stop bad changes in immune cells caused by a harmful substance, which could help prevent heart problems.
Methodology
Healthy monocytes were treated with OxLDL, with or without Mdivi-1 or S1QEL, and assessed for metabolic shifts, inflammatory cytokine expression, foam cell formation, and ROS production.
Limitations
In vitro results may not completely represent outcomes in a more complex in vivo setting.
Participant Demographics
Healthy, non-obese young adults aged 18 to 40.
Statistical Information
P-Value
p=0.006, p=0.027, p=0.025, p=0.008, p<0.001, p=0.0395, p=0.021
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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