Potentiation of Mitogenic Activity of Platelet-Derived Growth Factor by Physiological Concentrations of Insulin via the MAP Kinase Cascade in Rat A10 Vascular Smooth Muscle Cells
2002
Insulin Enhances Growth Factor Activity in Vascular Cells
publication
Evidence: moderate
Author Information
Author(s): HITOMI YAMADA, TOSHIO TSUSHIMA, HITOMI MURAKAMI, YASUKO UCHIGATA, YASUHIKO IWAMOTO
Primary Institution: Tokyo Women's Medical University
Hypothesis
Does insulin affect the mitogenic signaling induced by platelet-derived growth factor (PDGF) in rat vascular smooth muscle cells?
Conclusion
Insulin at physiological concentrations enhances PDGF-stimulated DNA synthesis in vascular smooth muscle cells through the MAPK signaling pathway.
Supporting Evidence
- Insulin alone has little mitogenic activity but enhances PDGF-stimulated DNA synthesis.
- Insulin potentiates the activation of the MAPK cascade stimulated by PDGF.
- The synergistic effect of insulin on DNA synthesis is not observed when MAPK is inhibited.
Takeaway
Insulin helps cells grow by making a growth factor work better, which is important for understanding heart disease.
Methodology
The study used rat A10 vascular smooth muscle cells to assess the effects of insulin on PDGF-induced DNA synthesis and signaling pathways.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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