TEL Represses Gene Activation by Binding to RAR and RXR
Author Information
Author(s): Magda A. Meester-Smoor, Marjolein J. F. W. ter Haar, W. Martijn van Wely, Karel H. M. Aarnoudse, Albert-Jan L. H. J. van Oord, Gertine van Tilburg, Gabrielle B. A. Zwarthoff, Ellen C. Z. van der Plas
Primary Institution: Erasmus MC, Rotterdam, The Netherlands
Hypothesis
TEL acts as a transcriptional repressor of RAR signaling by binding to RAR and RXR.
Conclusion
TEL is identified as a co-repressor of RAR-RXR-mediated transcription, functioning independently of histone deacetylases.
Supporting Evidence
- TEL binds directly to RARα and RXRα, indicating its role in transcriptional repression.
- The shorter isoform of TEL is a more efficient repressor of RAR signaling compared to the longer isoform.
- Repression by TEL does not involve recruitment of histone deacetylases, suggesting alternative mechanisms.
- Both HLH and DBD domains of TEL are crucial for its binding to RARα.
Takeaway
TEL is a protein that helps turn off certain genes by sticking to other proteins called RAR and RXR, which are important for cell growth and development.
Methodology
The study involved binding assays, transfection experiments in Hep3B cells, and luciferase reporter assays to assess transcriptional activity.
Limitations
The study does not explore the full range of biological processes influenced by TEL and RAR signaling.
Digital Object Identifier (DOI)
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